Simultaneous detection of inflammatory process indicators via operando dual lossy mode resonance-based biosensor
基于操作双损耗模式共振的生物传感器同时检测炎症过程指标
オペランド二重ロスモード共振に基づくバイオセンサーによる炎症プロセス指標の同時検出
operando 이중 손실 모드 공진 기반 바이오센서를 통한 염증 과정 지표의 동시 검출
Detección simultánea de indicadores del proceso inflamatorio mediante biosensor basado en resonancia de modo perdido dual operando
Détection simultanée des indicateurs du processus inflammatoire via un biosenseur basé sur la résonance de mode à perte double en fonctionnement simultané
Одновременное обнаружение показателей воспалительного процесса с помощью биосенсора на основе резонанса с двойным потерянным режимом в работе
Desiree Santano ¹, Abian B. Socorro ¹ ², Ambra Giannetti ³, Ignacio Del Villar ¹ ², Francesco Chiavaioli ³
¹ Electrical and Electronic Engineering Department, Public University of Navarra, Pamplona 31006, Spain
² Institute of Smart Cities (ISC), Public University of Navarra, Pamplona 31006, Spain
³ Institute of Applied Physics "Nello Carrara" (IFAC), National Research Council of Italy (CNR), Sesto Fiorentino 50019, Italy
Detecting multiple analytes simultaneously, crucial in disease diagnosis and treatment prognosis, remains challenging. While planar sensing platforms demonstrate this capability, optical fiber sensors still lag behind. An operando dual lossy mode resonance (LMR) biosensor fabricated on a D-shaped single-mode fiber (SMF) is proposed for quantification of clinical indicators of inflammatory process, like in COVID-19 infection.
Dual LMRs, created via two-step deposition process, yield a nanostructure with distinct SnO2 thicknesses on the flat surface of the fiber. Theoretical and experimental analyses confirm its feasibility, showing a sensitivity around 4500 nm/RIU for both LMRs. A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays, showcasing selectivity for different immunoglobulins.
Real-time and label-free detection of two inflammatory markers, C-reactive protein and D-dimer, empowers the platform capability with a minimum detectable concentration below 1 μg/mL for both biomolecules, which is of clinical interest. This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection, presenting a novel, compact and multi-functional analytical tool.
