Local renin angiotensin system and sperm DNA fragmentation
局部肾素血管紧张素系统和精子 DNA 断裂
국소 레닌 안지오텐신 시스템 및 정자 DNA 단편화
Sistema local de renina angiotensina y fragmentación del ADN espermático
Système local rénine-angiotensine et fragmentation de l'ADN des spermatozoïdes
Локальная ренин-ангиотензиновая система и фрагментация ДНК сперматозоидов
María Victoria Aparicio Prieto ¹, María Victoria Rodríguez Gallego ², Asier Valdivia Palacín ³, Yosu Franco Iriarte ⁴, Gotzone Hervás Barbara ³, Enrique Echevarría Orella ³, Luis Casis Saenz ³
¹ Human Reproduction Unit, Cruces University Hospital, Barakaldo 48903, Spain
² Human Reproduction Unit, San Pedro Hospital, Logroño 26006, Spain
³ Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Leioa 48940, Spain
⁴ Human Reproduction Unit, Ruber International Hospital, Madrid 28034, Spain
Asian Journal of Andrology, 7 September 2021

The renin angiotensin system (RAS) appears to influence male fertility at multiple levels. In this work, we analyzed the relationship between the RAS and DNA integrity. Fifty male volunteers were divided into two groups (25 each): control (DNA fragmentation ≤20%) and pathological (DNA fragmentation >20%) cases.

Activities of five peptidases controlling RAS were measured fluorometrically: prolyl endopeptidase (which converts angiotensin [A] I and A II to A 1–7), neutral endopeptidase (NEP/CD10: A I to A 1–7), aminopeptidase N (APN/CD13: A III to A IV), aminopeptidase A (A II to A III) and aminopeptidase B (A III to A IV). Angiotensin-converting enzyme (A I to A II), APN/CD13 and NEP/CD10 were also assessed by semiquantitative cytometry and quantitative flow cytometry assays, as were the receptors of all RAS components: A II receptor type 1 (AT1R), A II receptor type 2 (AT2R), A IV receptor (AT4R or insulin-regulated aminopeptidase [IRAP]), (pro)renin receptor (PRR) and A 1–7 receptor or Mas receptor (MasR) None of the enzymes that regulate levels of RAS components, except for APN/CD13 (decrease in fragmented cells), showed significant differences between both groups.

Micrographs of RAS receptors revealed no significant differences in immunolabeling patterns between normozoospermic and fragmented cells. Labeling of AT1R (94.3% normozoospermic vs 84.1% fragmented), AT4R (96.2% vs 95.3%) and MasR (97.4% vs 87.2%) was similar between the groups. AT2R (87.4% normozoospermic vs 63.1% fragmented) and PRR (96.4% vs 48.2%) were higher in non-fragmented spermatozoa. These findings suggest that fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.
Asian Journal of Andrology_1
Asian Journal of Andrology_2
Asian Journal of Andrology_3
Asian Journal of Andrology_4
Reviews and Discussions
An Annotated List of Lizards (Sauria: Squamata) Recorded from the People’s Republic of China
Pyoderma gangrenosum in ulcerative colitis
Disordered Translocation is Hastening Local Extinction of the Chinese Giant Salamander
Literature review: non-intubated (tubeless) VATS for lung volume reduction surgery
Non-intubated thoracic surgery: an Asian perspective
A case of infectious endophthalmitis misdiagnosed as uveitis
A narrative review of glaucoma screening from fundus images
Structured eigenvalue problems in electronic structure methods from a unified perspective
Transmission, viral kinetics and clinical characteristics of the emergent SARS-CoV-2 Delta VOC in Guangzhou, China
Multiple-quantum-well-induced unipolar carrier transport multiplication in AlGaN solar-blind ultraviolet photodiode
In-band intermodulation induced by transient response of erbium-doped fiber amplifier
Buildup dynamics of multiple solitons in spatiotemporal mode-locked fiber lasers

Previous Article                                Next Article
Copyright © Hot Paper