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Continuous subcellular resolution three-dimensional imaging on intact macaque brain
完整猕猴脑的连续亚细胞分辨率三维成像
無傷のマカクの脳の連続細胞内解像度3次元イメージング
온전한 원숭이 뇌에 대한 지속적인 세포내 해상도 3차원 영상
Imágenes tridimensionales de resolución subcelular continua en cerebro de macaco intacto
Imagerie tridimensionnelle à résolution subcellulaire continue sur cerveau de macaque intact
Трехмерное изображение с непрерывным субклеточным разрешением на интактном мозге макака
Can Zhou 周灿 ¹, Xiaoquan Yang 杨孝全 ¹ ², Shihao Wu ³, Qiuyuan Zhong 钟秋园 ¹, Ting Luo ¹, Anan Li 李安安 ¹ ² ⁴, Guangcai Liu ¹, Qingtao Sun 孙庆涛 ², Pan Luo 罗盘 ¹, Lei Deng 邓磊 ¹, Hong Ni 倪鸿 ¹, Chaozhen Tan ¹, Jing Yuan 袁菁 ¹ ², Qingming Luo 骆清铭 ⁵, Xintian Hu 胡新天 ³ ⁴, Xiangning Li 李向宁 ¹ ², Hui Gong 龚辉 ¹ ² ⁴
¹ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China 华中科技大学 机械科学与工程学院 生物医学光子学教育部重点实验室 武汉光电国家实验室 Britton Chance 生物医学光子学研究中心
² HUST-Suzhou Institute for Brainsmatics, Suzhou 215123, China 华中科技大学苏州脑空间信息研究院
³ Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China 中国科学院 昆明动物研究所 动物模型与人类疾病机理重点实验室
⁴ CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Science, Shanghai 200031, China 中国科学院 脑科学与智能技术卓越创新中心
⁵ School of Biomedical Engineering, Hainan University, Haikou 570228, China 海南大学 生物医学工程学院
Science Bulletin, 5 August 2021
Abstract

To decipher the organizational logic of complex brain circuits, it is important to chart long-distance pathways while preserving micron-level accuracy of local network. However, mapping the neuronal projections with individual-axon resolution in the large and complex primate brain is still challenging.

Herein, we describe a highly efficient pipeline for three-dimensional mapping of the entire macaque brain with subcellular resolution. The pipeline includes a novel poly-N-acryloyl glycinamide (PNAGA)-based embedding method for long-term structure and fluorescence preservation, high-resolution and rapid whole-brain optical imaging, and image post-processing. The cytoarchitectonic information of the entire macaque brain was acquired with a voxel size of 0.32 × 0.32 × 10 μm³, showing its anatomical structure with cell distribution, density, and shape.

Furthermore, thanks to viral labeling, individual long-distance projection axons from the frontal cortex were for the first time reconstructed across the entire brain hemisphere with a voxel size of 0.65 × 0.65 × 3 μm³. Our results show that individual cortical axons originating from the prefrontal cortex simultaneously target multiple brain regions, including the visual cortex, striatum, thalamus, and midbrain. This pipeline provides an efficient method for cellular and circuitry investigation of the whole macaque brain with individual-axon resolution, and can shed light on brain function and disorders.
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