MMM
YYYY
p62/SQSTM1 Participates in the Innate Immune Response of Macrophages Against Candida albicans Infection
p62/SQSTM1 参与巨噬细胞对白色念珠菌感染的先天免疫反应
p62 / SQSTM1は、カンジダアルビカンス感染に対するマクロファージの自然免疫応答に関与します
p62/SQSTM1은 칸디다 알비칸스 감염에 대한 대식세포의 타고난 면역 반응에 참여합니다
p62/SQSTM1 participa en la respuesta inmune innata de los macrófagos contra la infección por Candida albicans
p62/SQSTM1 participe à la réponse immunitaire innée des macrophages contre l'infection à Candida albicans
p62/SQSTM1 участвует во врожденном иммунном ответе макрофагов против инфекции Candida albicans
He Yan-Zhi ¹, Duan Zhi-Min 段志敏 ², Chen Xu 陈旭 ¹ ³, Li Min 李岷 ¹ ³
¹ Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China
中国 南京 南京医科大学 公共卫生学院 全球健康中心
² Department of Dermatology, Hospital for Skin Diseases (Institute of Dermatology), Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu 210042, China
中国 江苏 南京 中国医学科学院 北京协和医学院 皮肤病医院(皮肤病研究所)皮肤科
³ Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Hospital for Skin Diseases (Institute of Dermatology), Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu 210042, China
中国 江苏 南京 中国医学科学院 北京协和医学院 皮肤病医院(皮肤病研究所)江苏省皮肤病与性病分子生物学重点实验室
International Journal of Dermatology and Venereology, 1 June 2022
Abstract

Objective

This study was designed to evaluate whether p62/SQSTM1 (hereafter referred to as p62) is involved in the immune response of macrophages against challenge by Candida albicans (C. albicans).

Methods

We cultured bone marrow-derived macrophages (BMDMs) to investigate the immune response to challenge by C. albicans. The p62 gene was knocked down by transfection with p62 small interfering RNA (siRNA) in the p62 siRNA group. BMDMs transfected with nonsense siRNA served as the negative control (NC) group. These two groups of BMDMs were challenged with C. albicans in vitro. We detected p62 expression through quantitative reverse transcription PCR and western blotting. The phagocytosis ability of BMDMs was evaluated by flow cytometry and microscopic examination using an Olympus FV1000 laser scanning confocal microscope. Moreover, we determined the level of reactive oxygen species (ROS) in BMDMs. The mRNA levels of proinflammatory cytokines were determined by quantitative reverse transcription PCR.

Results

After stimulation by C. albicans, the relative expression of p62 mRNA was increased in a dose-dependent manner, the relative expression of p62 and the ratio of BMDMs to C. albicans is 1.893 ± 0.2156 (1:1, P < 0.05), 2.873 ± 0.4787 (1:3, P < 0.05) and 3.556 ± 0.2892 (1:5, P < 0.01). The p62 protein level was also increased. After transfection with p62 siRNA, the mRNA and protein levels of p62 were significantly decreased in BMDMs (P < 0.05). After 0.5, 1 and 2 hours of co-culture of BMDMs with C. albicans, flow cytometry showed that the phagocytosis rates of C. albicans by BMDMs were significantly lower in the p62 siRNA group than in the NC group (39.70 ± 1.69% vs. 55.23 ± 0.72%, 46.70 ± 0.89% vs. 60.80 ± 1.78%, 51.90 ± 0.98% vs. 64.43 ± 2.0%, respectively, all P < 0.05). Consistent results were seen in the production of ROS (4269 ± 392.6 vs. 13426 ± 1859.7, 4967 ± 721.2 vs. 13687 ± 2611.2, 7647 ± 1950.0 vs. 17719 ± 1814.2, respectively, all P < 0.05). The ROS levels were higher in BMDMs of the NC group than in BMDMs transfected with p62 siRNA at 0.5, 1, and 2 hours after treatment with C. albicans. BMDMs was co-cultured with C. albicans for 4 and 12 hours, the mRNA levels of interleukin-1β and interleukin-18 in NCs were also higher than p62 siRNA group, interleukin-1β: (6.14 ± 1.63 vs. 12.12 ± 0.54, 8.81 ± 0.86 vs. 26.2 ± 4.67, respectively, all P < 0.05), IL-18: (0.38 ± 0.02 vs. 0.97 ± 0.06, 0.44 ± 0.02 vs. 2.23 ± 0.46, respectively, all P < 0.05).

Conclusion

p62 plays an important role in the process of phagocytosis in BMDMs challenged by C. albicans through ROS production and expression of proinflammatory cytokines.
International Journal of Dermatology and Venereology_1
International Journal of Dermatology and Venereology_2
International Journal of Dermatology and Venereology_3
International Journal of Dermatology and Venereology_4
International Journal of Dermatology and Venereology_5
Reviews and Discussions
https://www.hotpaper.io/index.html
Perovskite-transition metal dichalcogenides heterostructures: recent advances and future perspectives
Functional isolation, culture and cryopreservation of adult human primary cardiomyocytes
Ultrahigh-resolution on-chip spectrometer with silicon photonic resonators
Tracing the formation of oxygen vacancies at the conductive LaAlO₃/SrTiO₃ interface via photoemission
Photonic spin Hall effect: fundamentals and emergent applications
Research on Visual Detection Algorithm for Groove Feature Sizes by Means of Structured Light Projection
Active odd-mode-metachannel for single-conductor systems
Dual bound states in the continuum enhanced second harmonic generation with transition metal dichalcogenides monolayer
An overview on the principle of inkjet printing technique and its application in micro-display for augmented/virtual realities
Time resolved studies reveal the origin of the unparalleled high efficiency of one nanosecond laser ablation in liquids
Nonlinear optics with structured light
Relationship between adjustment of low water level and utilization of water depth in Shashi Reach in middle Yangtze River



Previous Article                                Next Article
About
|
Contact
|
Copyright © Hot Paper