A virus-derived small RNA targets the rice transcription factor ROC1 to induce disease-like symptom
一种病毒衍生的小 RNA 靶向水稻转录因子 ROC1 以诱导疾病样症状
ウイルス由来の低分子RNAは、イネの転写因子ROC1を標的にして、病気のような症状を誘発します
바이러스 유래 작은 RNA가 벼 전사인자 ROC1을 표적으로 하여 질병 유사 증상 유발
Un pequeño ARN derivado de un virus se dirige al factor de transcripción del arroz ROC1 para inducir un síntoma similar a una enfermedad
Un petit ARN dérivé du virus cible le facteur de transcription du riz ROC1 pour induire des symptômes de type maladie
Малая РНК, полученная из вируса, нацелена на фактор транскрипции риса ROC1, вызывая симптомы, подобные болезни
¹ Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, 510642, China
中国 广州 华南农业大学植物保护学院 广东省微生物信号与作物病害防控重点实验室
² School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, 215123, China
中国 苏州 苏州大学基础医学与生物科学学院
Virus-derived small interfering RNAs (vsiRNAs) in plants can target host transcripts to induce symptom development. Southern rice black-streaked dwarf virus (SRBSDV) is the most damaging rice-infecting virus, which causes severe stunting and poorly developed roots in rice plants, thereby posing a serious threat to rice production.
In this study, we show that a vsiRNA (vsiR-S9-18), generated from SRBSDV genome segment 9, targets the transcription factor ROC1 in rice. SRBSDV infection triggered the production of vsiR-S9-18 and resulted in the downregulation of ROC1 in rice plants. Transient expression of vsiR-S9-18 in rice protoplasts and tobacco leaves further demonstrated the specific association of vsiR-S9-18 with ROC1.
Moreover, the ROC1-knockout rice plants displayed shortened roots, similar to the phenotype of root growth inhibition caused by SRBSDV infection. We propose that vsiR-S9-18 inhibits root elongation via ROC1, which has been proven to regulate root growth. This finding extends our understanding of the role of vsiRNA in viral disease development and is helpful for the development of new antiviral approaches.
