Functional divergence of Populus MYB158 and MYB189 gene pair created by whole genome duplication
全基因组复制产生的杨树MYB158和MYB189基因对的功能差异
全ゲノム重複によって作成されたポプラMYB158およびMYB189遺伝子ペアの機能的相違
전체 게놈 복제에 의해 생성된 Populus MYB158 및 MYB189 유전자 쌍의 기능적 발산
Divergencia funcional del par de genes Populus MYB158 y MYB189 creado por la duplicación del genoma completo
Divergence fonctionnelle de la paire de gènes Populus MYB158 et MYB189 créée par duplication du génome entier
Функциональная дивергенция пары генов Populus MYB158 и MYB189, созданная путем дупликации всего генома
¹ State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
中国 北京 中国科学院植物研究所 系统与进化植物学国家重点实验室
² University of Chinese Academy of Sciences, Beijing 100049, China
中国 北京 中国科学院大学
³ State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
中国 北京 中国林业科学研究院林木遗传育种国家重点实验室
⁴ College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
中国 北京 北京林业大学生物科学与技术学院
⁵ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
中国 广州 华南农业大学林学与风景园林学院 广东省森林植物种质创新与利用重点实验室
Journal of Systematics and Evolution, 1 January 2022
Abstract
Whole genome duplication (WGD) provides new genetic material for genome evolution. After a WGD event, some duplicates are lost, while other duplicates still persist and evolve diverse functions. A particular challenge is to understand how this diversity arises.
This study identified two WGD-derived duplicates, MYB158 and MYB189, from Populus tomentosa. Populus MYB158 and MYB189 had expression divergence. Populus tomentosa overexpressing MYB158 or MYB189 had similar phenotypes: creep growth, decreased width of xylem and secondary cell wall thickness.
Compared to wild-type, neither myb158 mutant nor myb158 myb189 double mutant showed obvious phenotypic variation in P. tomentosa. Although MYB158 and MYB189 proteins could repress the same structural genes involved in lignin, cellulose, and xylan biosynthesis, the two proteins had their own specific regulatory targets. Populus MYB158 could act as the upstream regulator of secondary cell wall NAC master switch and directly represses the expression of the SND1-B2 gene.
Taken together, Populus MYB158 and MYB189 have retained similar functions in negatively regulating secondary cell wall biosynthesis, but have evolved partially distinct functions in direct regulation of NAC master switch, with MYB158 playing a more crucial role. Our findings provide new insights into the evolutionary and functional divergence of WGD-derived duplicate genes.
