Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks
整合肝脏蛋白质组学和代谢组学研究广泛酸缺乏对北京鸭肝脏代谢通路的影响
総合肝臓蛋白質群学と代謝群学による北京鴨汎酸欠乏影響の代謝経路の確定
간 단백질 그룹학 과 대사 그룹학 을 종합하여 베이징 오리 범산 결핍 영향 의 대사 경로 를 확정 하다
Combinación de proteómica hepática y Metabolómica para determinar las vías metabólicas de los efectos de la deficiencia de ácido pantoténico en los patos de Beijing
Protéomique hépatique intégrée et Métabolomique identification des voies métaboliques affectées par la carence en acide pantothénique canard de Pékin
Интегрированная протеомика печени и метаболизм определяют метаболические пути воздействия дефицита панкислоты у утки в Пекине
Jing Tang 唐静, Yongbao Wu 吴永保, Bo Zhang 张博, Suyun Liang 梁素芸, Zhanbao Guo 郭占宝, Jian Hu 胡健, Zhengkui Zhou 周正奎, Ming Xie 谢明, Shuisheng Hou 侯水生
State Key Laboratory of Animal Nutrition, Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193, Beijing, China
中国 北京 中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室 农业农村部动物遗传育种与繁殖（家禽）重点实验室
Pantothenic acid deficiency (PAD) in animals causes growth depression, fasting hypoglycemia and impaired lipid and glucose metabolism. However, a systematic multi-omics analysis of effects of PAD on hepatic function has apparently not been reported. We investigated liver proteome and metabolome changes induced by PAD to explain its effects on growth and liver metabolic disorders. Pekin ducks (1-d-old, n = 128) were allocated into 2 groups, with 8 replicates and 8 birds per replicate.
For 16 d, all ducks had ad libitum access to either a PAD or a pantothenic acid adequate (control, CON) diet, formulated by supplementing a basal diet with 0 or 8 mg pantothenic acid/kg of diet, respectively. Liver enlargement, elevated liver glycogen concentrations and decreased liver concentrations of triglyceride and unsaturated fatty acids were present in the PAD group compared to the CON group.
Based on integrated liver proteomics and metabolomics, PAD mainly affected glycogen synthesis and degradation, glycolysis and gluconeogenesis, tricarboxylic acid (TCA) cycle, peroxisome proliferator-activated receptor (PPAR) signaling pathway, fatty acid beta oxidation, and oxidative phosphorylation. Selected proteins were confirmed by Western blotting. Downregulation of proteins and metabolites involved in glycogen synthesis and degradation, glycolysis and gluconeogenesis implied that these processes were impaired in PAD ducks, which could have contributed to fasting hypoglycemia, liver glycogen storage, insufficient ATP production, and growth retardation.
In contrast, PAD also upregulated proteins and metabolites involved in fatty acid beta oxidation, the TCA cycle, and oxidative phosphorylation processes in the liver; presumably compensatory responses to produce ATP. We inferred that PAD decreased liver triglyceride and unsaturated fatty acids by activating fatty acid beta oxidation and impairing unsaturated fatty acid synthesis. These findings contributed to our understanding of the mechanisms of PAD-induced changes in hepatic metabolism.
