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Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution
用于中尺度和亚微米分辨率的多波长显微镜的大视场物镜
メソスケールとサブミクロン分解能のための多波長顕微鏡用の大視野対物レンズ
중척도와 마이크로미터 해상도의 다파장 현미경을 위한 대시물경
Lentes de gran campo de visión para microscopios multilongitud de onda de resolución mesoescala y submicron
Objectifs grand champ pour microscopes Multi - longueurs d'onde à résolution mésométrique et submicronique
Объект с большим полем зрения для многоволновых микроскопов со средним и субмикронным разрешением
Xin Xu ¹ ², Qin Luo ¹ ², Jixiang Wang ¹ ², Yahui Song ¹ ², Hong Ye ², Xin Zhang ², Yi He ², Minxuan Sun ¹ ², Ruobing Zhang ¹ ², Guohua Shi ¹ ²
¹ School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
中国 合肥 中国科大生物医学工程学院(苏州)
² Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou 215163, China
中国 苏州 中国科学院苏州生物医学工程技术研究所 江苏省医用光学重点实验室
Opto-Electronic Advances, 11 June 2024
Abstract

Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view, typically around 1 mm. This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample, such as different brain areas. In addition, conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.

Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm, a numerical aperture of 0.5, and a working wavelength range from 400 to 1000 nm. We achieved a resolution of 0.74 μm in fluorescent beads imaging. The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system, a confocal laser scanning system, and a two-photon imaging system.

This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.
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