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All-fiber-transmission photometry for simultaneous optogenetic stimulation and multi-color neuronal activity recording
用于同时光遗传学刺激和多色神经元活动记录的全光纤传输光度法
光遺伝学的刺激とマルチカラー神経活動記録を同時に行うための全ファイバー透過測光
동시 광유전학적 자극 및 다색 뉴런 활동 기록을 위한 전체 섬유 투과 광도계
Fotometría de transmisión de fibra para estimulación optogenética simultánea y registro de actividad neuronal multicolor
Photométrie à transmission toutes fibres pour une stimulation optogénétique simultanée et un enregistrement multicolore de l'activité neuronale
Полноволоконная фотометрия для одновременной оптогенетической стимуляции и многоцветной регистрации активности нейронов
Zhongyang Qi 齐中阳 ¹ ² ³, Qingchun Guo 郭青春 ⁴ ⁵ ⁶, Shu Wang ⁶, Mingyue Jia ⁶, Xinwei Gao ⁶, Minmin Luo 罗敏敏 ³ ⁶ ⁷, Ling Fu 付玲 ¹ ²
¹ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
中国 武汉 华中科技大学武汉光电国家研究中心 Britton Chance 生物医学光子学研究中心
² MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China
中国 武汉 华中科技大学工程科学学院 生物医学光子学教育部重点实验室
³ National Institute of Biological Sciences, Beijing 102206, China
中国 北京 北京生命科学研究所
⁴ Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beijing 100191, China
中国 北京 大数据精准医疗高精尖创新中心
⁵ School of Biomedical Engineering, Capital Medical University, Beijing 100069, China
中国 北京 首都医科大学生物医学工程学院
⁶ Chinese Institute for Brain Research, Beijing 102206, China
中国 北京 北京脑科学与类脑研究中心
⁷ School of Life Sciences, Tsinghua University, Beijing 100084, China
中国 北京 清华大学生命科学学院
Opto-Electronic Advances, 20 May 2022
Abstract

Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuitsin vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently.

Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths.

Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine (DA) responses to unexpected rewards in the nucleus accumbens (NAc) in a freely moving mouse.

We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.
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